Xenbase Image ID: 174542

Figure 5. The paraxial myogenic mesoderm is mispatterned in fgfr4 CRISPR embryos. (A–F, G–J) Dorsal-vegetal views showing expression of an array of mesodermal markers in stage 10 (A–F) and stage 12 (G–J) embryos. Expression of paraxial mesoderm markers myf5 and myoD is perturbed in fgfr4 CRISPR embryos. (K, L) Xbra expression in embryos bisected through their dorsal midline at stages 10.5 and 12; red arrowheads point at xbra expression in the involuted mesoderm. Graphs show percentages of embryos with normal vs. abnormal expression of each marker; N = 32–40 embryos per Control or fgfr4 CRISPR; **p < 0.001, ***p < 0.0001. Image published in: Sempou E et al. (2018) Copyright © 2018 Sempou, Lakhani, Amalraj and Khokha. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue gsc goosecoid, gsc-a, gsc-b, Xgsc X. tropicalis Throughout NF stage 10 mesoderm nodal3.1 nr3, Xnr-3, Xnr3 X. tropicalis Throughout NF stage 10 mesoderm foxj1 foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 X. tropicalis Throughout NF stage 10 mesoderm ventx2 ventx2.1, ventx2.1-a, ventx2.1-b, vox, vox1, Xbr-1a, xvent-2, xvent-2b, Xvr-11b X. tropicalis Throughout NF stage 10 mesoderm myf5 myf-5, Xmyf-5, Xmyf5 X. tropicalis Throughout NF stage 10 mesoderm tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. tropicalis Throughout NF stage 10 mesoderm tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. tropicalis Throughout NF stage 12 circumblastoporal collar presumptive axial mesoderm myod1 mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. tropicalis Throughout NF stage 12 presumptive paraxial mesoderm myf5 myf-5, Xmyf-5, Xmyf5 X. tropicalis Throughout NF stage 12 presumptive paraxial mesoderm foxj1 foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 X. tropicalis Throughout NF stage 12 upper blastopore lip Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + fgfr4 CRISPR + NF10 (in situ hybridization) Fig. 5 E Disease congenital heart disease visceral heterotaxy Expression Phenotype decreased amount myf5 expression in mesoderm Xtr Wt + fgfr4 CRISPR + NF12 (in situ hybridization) Fig. 5 H Disease congenital heart disease visceral heterotaxy Expression Phenotype decreased amount myf5 expression in presumptive paraxial mesoderm Xtr Wt + fgfr4 CRISPR + NF12 (in situ hybridization) Fig. 5 I Disease congenital heart disease visceral heterotaxy Expression Phenotype decreased amount myod1 expression in presumptive paraxial mesoderm Xtr Wt + fgfr4 CRISPR + NF12 (in situ hybridization) Fig. 5 L Disease congenital heart disease visceral heterotaxy Expression Phenotype decreased amount tbxt expression in presumptive paraxial mesoderm Larger Image Printer Friendly View

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