Xenbase Image ID: 174601

Fig. 3. Lif signaling in Xenopus embryo. (A) 5 or 100 pg of lif mRNA were injected in two cell-stage embryos and Stat3 phosphorylation status (α-Tyr705, top) was analysed by western blot analysis in stage 12 or 27 injected embryos. The total content of Stat3 protein was revealed by anti α-Stat3 antibody (bottom). Uninjected embryos are used as control. (B) Immunofluorescence analysis of P-Stat3 in lif mRNA-injected embryo. Transverse section of stage 22 embryo with dorsal side on the top (a) was analysed for immunostaining of P-Stat3 (c, red) and DAPI (b, blue). b and c are magnification of the white square in a. Arrows indicate representative P-Stat3 cells. Scale bar represents 50 µm. (C) Western blot analysis of stage 12 embryos injected with 20 pg of lif mRNA at the two cell stage with 2000 pg of δlifr mRNA using anti-phosphorylated Tyr705 Stat3 (α-Tyr705, top) and anti-Stat3 (α-Stat3, bottom) antibodies. Uninjected embryos are used as control. Relative quantification of phospho-Stat3 (P-Stat3) is displayed on the right. The diagram represents the mean of three independent measurements with standard deviations. Image published in: Jalvy S et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + lif + NF12 (western blot) Fig. 3 A Anatomical Phenotype abnormal protein phosphorylation Xla Wt + lif + NF27 (western blot) Fig. 3 A Anatomical Phenotype abnormal protein phosphorylation Xla Wt + lif + NF22 (immunohistochemistry) Anatomical Phenotype abnormal protein phosphorylation Larger Image Printer Friendly View

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