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Fig. 4. Lif activation of target genes. Real-time RT-PCR analysis of lif mRNA injected embryos. Embryos were either uninjected (-) or injected with 20 pg of lif mRNA (lif) cultured until stage 12.5 and processed for RT-qPCR of the indicated genes. Five embryos were used in each lot. All values were normalized and calibrated to the expression of the reference gene odc. Bar plots show the mean of three independent ± SEM of normalized fold induction compared to uninjected control embryos. Image published in: Jalvy S et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 1 col 1 Expression Phenotype increased amount socs3.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 1 col 2 Expression Phenotype increased amount fos.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 1 col 3 Expression Phenotype increased amount junb.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 1 col 4 Expression Phenotype increased amount myc.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 2 col 1 Expression Phenotype increased amount zfp36.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 2 col 2 Expression Phenotype increased amount klf4.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 2 col 3 Expression Phenotype increased amount klf5.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 4 row 2 col 4 Expression Phenotype increased amount pou5f3.1.L expression in embryo Larger Image Printer Friendly View

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