Xenbase Image ID: 174603

Fig. 5. Lif overexpression in Xenopus embryo causes ventralization and anterior truncation. (A) Two-cell stage embryos were injected in each blastomere at the animal pole with 2, 20 or 200 pg of lif mRNA (+lif). Representative phenotypes observed at stage (St) 18, 22 or 33–34 are shown (anterior view for St18 embryos, lateral view and anterior to the left with dorsal to the top for St 22 and St 33–34 embryos). (-lif) uninjected embryos. (B). Embryos injected with 20 pg of lif mRNA were fixed at late neurula stage and analysed by in situ hybridization for the expression of the indicated genes. Embryos are observed on lateral view with anterior to the left and dorsal to the top. Insets are anterior view of the embryos. (C) RT-qPCR analysis of lif mRNA injected embryos (lif) versus uninjected control embryos (-). Embryos were injected with 20 pg of lif mRNA and cultured until stage 22 before processing for RT-qPCR. Five embryos were used in each lot. All values were normalized and calibrated to the expression of the reference gene odc. Bar plots show the mean ±SEM of at least three independent experiments. Image published in: Jalvy S et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 13 to NF stage 21 anterior neural tube egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 13 to NF stage 21 presumptive rhombomere vgll3.L LOC108709205 X. laevis Throughout NF stage 13 to NF stage 21 presumptive rhombomere pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 13 to NF stage 21 eye primordium neural tube Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + lif + NF 18 (whole mount microscopy) Fig. 5 A col 1 Anatomical Phenotype decreased width of the neural plate Xla Wt + lif + NF 22 (whole mount microscopy) Fig. 5 A col 2 Anatomical Phenotype abnormal anterior/posterior axis specification abnormally ventralized embryo Xla Wt + lif + NF 33 - NF 34 (whole mount microscopy) Fig. 5 A col 3 Anatomical Phenotype abnormal anterior/posterior axis specification abnormally ventralized embryo absent head Xla Wt + lif + NF 18- NF 21 (in situ hybridization) Fig. 5 B row 1 col 2 Expression Phenotype decreased amount otx2.L expression in forebrain decreased amount otx2.L expression in midbrain Xla Wt + lif + NF 18- NF 21 (in situ hybridization) Fig. 5 B row 2 col 2 Expression Phenotype decreased amount egr2.L expression in hindbrain decreased amount vgll3.L expression in hindbrain Xla Wt + lif + NF 18- NF 21 (in situ hybridization) Fig. 5 B row 3 col 2 Expression Phenotype decreased amount pax6.L expression in eye Xla Wt + lif + NF 22 (RT-PCR) Fig. 5 C Expression Phenotype decreased amount egr2.L expression in embryo decreased amount otx2.L expression in embryo decreased amount pax6.L expression in embryo decreased amount vgll3.L expression in embryo Larger Image Printer Friendly View

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