Xenbase Image ID: 174605

Fig. 7. Lif overexpression upregulates BMP signaling and antagonizes IGF signal. (A) Embryos were either not injected (-) or injected with 20 pg of lif mRNA (lif) at the two-cell stage. They were cultured until stage 12.5 and processed for RT-qPCR analysis. (B) Embryos were injected with 1 ng of igf1 mRNA (igf) or with 1 ng of igf1 together with 20 pg of lif mRNA (igf+lif). Animal caps were explanted at blastula stage and cultured until stage 18 before RT-qPCR analysis. (C) Embryos were either not injected (-) or injected with 20 pg of lif mRNA (lif). They were cultured until stage 18 and processed for RT-qPCR analysis. Soc3 was used as a control of lif signaling activation. Five embryos or 15–20 caps were used in each lot. All values were normalized and calibrated to the expression of the reference gene odc. Bar plots show the mean ±SEM of at least three independent experiments. Image published in: Jalvy S et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 7 A row 1 col 1 Expression Phenotype decreased amount cer1.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 7 A row 1 col 2 Expression Phenotype decreased amount dkk1.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 7 A row 1 col 2 Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 7 A row 1 col 3 Expression Phenotype increased amount msx1.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 7 A row 2 col 1 Expression Phenotype increased amount ventx1.2.L expression in embryo Xla Wt + lif + NF 12.5 (RT-PCR) Fig. 7 A row 2 col 2 Expression Phenotype increased amount ventx2.2.L expression in embryo Xla Wt + igf1 + animal explant + NF 18 (RT-PCR) Fig. 7 B col 1 Expression Phenotype increased amount otx2.L expression in animal cap Xla Wt + igf1 + animal explant + NF 18 (RT-PCR) Fig. 7 B col 2 Expression Phenotype increased amount pax6.L expression in animal cap Xla Wt + lif + NF 18 (RT-PCR) Fig. 7 C col 1 Expression Phenotype increased amount socs3.L expression in embryo Xla Wt + lif + NF 18 (RT-PCR) Fig. 7 C col 2 Expression Phenotype decreased amount igfbp5.L expression in embryo Larger Image Printer Friendly View

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