Xenbase Image ID: 174606

Fig. 8. Lif overexpression inXenopusembryo induces tumor-like masses formation. (A) Control uninjected stage 28 embryo (a) or stage 28 (b) and stage 33–34 (c) embryos injected with 20 pg of lif mRNA. Tumor-like mass are visible in ventral position (arrowhead). (B) 20 pg of lif mRNA and 250 pg of lacZ mRNA were co-injected in ventral (V1, V2) or dorsal (D1, D2) blastomeres of 8-cell stage embryos (schematic diagram). Developing embryos were fixed at stage 31 for β-galactosidase coloration. Tumor-like mass formed are indicated by arrow in embryos derived from targeted injections. (C) Histological analysis of embryos injected with 20 pg of lif mRNA. Tumor-like structures in black boxes in a and c are magnified in b and d respectively. (D) 20 pg of lif mRNA and 250 pg of GFP mRNA were co-injected in ventral (V2) or dorsal (D1) blastomeres of 8-cell stage embryos. Developing embryos were observed under white light (a, c, e, g) or fluorescent light (b, d, f, h). Tumor-like masses in white boxes are in dotted circle when observed under fluorescent light. g and h are magnification of e and f respectively. Image published in: Jalvy S et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + lif + NF 28 (whole mount microscopy) Fig. 8 A b Anatomical Phenotype increased cell population proliferation in ventral region Disease cancer Xla Wt + lif + NF 33 - NF 34 (whole mount microscopy) Fig. 8 A c Anatomical Phenotype increased cell population proliferation in ventral region Disease cancer Xla Wt + lif + NF 30 - NF 35 (whole-mount microscopy) Fig. 8 B col 1 row 1 Anatomical Phenotype increased cell population proliferation in antero-ventral region Disease cancer Xla Wt + lif + NF 30 - NF 35 (whole-mount microscopy) Fig. 8 B col 1 row 2 Anatomical Phenotype increased cell population proliferation in posterior region Disease cancer Xla Wt + lifr + NF 30 - NF 35 (whole-mount microscopy) Fig. 8 B col 2 row 1 Anatomical Phenotype increased cell population proliferation in antero-dorsal region Disease cancer Xla Wt + lif + NF 30 - NF 35 (whole-mount microscopy) Fig. 8 B col 2 row 2 Anatomical Phenotype increased cell population proliferation in postero-dorsal region Disease cancer Xla Wt + lif + NF 30 - NF 56 (histology) Fig. 8 C a-d Anatomical Phenotype abnormal epidermis morphology increased cell population proliferation in epidermis Disease cancer Xla Wt + lif + NF 30 - NF 35 (whole-mount microscopy) Fig. 8 D a-d Anatomical Phenotype increased cell population proliferation in trunk Disease cancer Xla Wt + lifr + NF 30 - NF 35 (whole-mount microscopy) Fig. 8 D e-h Anatomical Phenotype increased cell population proliferation in trunk Disease cancer Larger Image Printer Friendly View

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