Xenbase Image ID: 174615

Figure S7. Overexpression in Xenopus embryos of ctf1 or cntf has no effect on embryonic development. (A) Embryos were injected with 20 pg of lif mRNA or 2 ng of either ctf1 or cntf mRNAs. Embryos were observed at stage (St) 27 or 37-38 for phenotype alteration. (B) RNA extracted from stage 45 embryonic tissues was analysed by RT-PCR. Br, brain, He, heart; Ki, kidney; So, somites. E, total non-dissected embryo; - control without reverse transcription. Ornithine decarboxylase (odc) expression was used as control. Image published in: Jalvy S et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + lif + NF27-37/38 (whole-mount microscopy) Fig. S7 A Anatomical Phenotype abnormal anterior/posterior axis specification abnormally ventralized embryo decreased size of the head Larger Image Printer Friendly View

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