XB-IMG-174687
Xenbase Image ID: 174687
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Figure 6—figure supplement 1. Arid3a-photo-morpholino blocks the effect of Arid3a-antisense morpholino.(A) Sequences of arid3a.L-antisense-MO (arid3a.L-MO) and arid3a.L-Photo-MO. The upper panel shows a schematic illustration of arid3a-antisense-MO. Sequences show the target region of arid3a.L-MO and its sense arid3a.L-Photo-MO. (B) arid3a.L-MO causes developmental defects in early-stage embryos. arid3a.L-MO with venus-NLS mRNA is injected at the one-cell stage. (C) The arid3a.L-MO blocks the splicing of the arid3a transcript. Total RNA was purified from control and arid3a.L-MO-injected embryos, and RT-PCR analysis was performed. Lanes 1–3: three independent experiments. (D) Embryos injected with arid3a.L-MO inactivated by arid3a.L-Photo-MO developed normally. Image published in: Suzuki N et al. (2019) © 2019, Suzuki et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |