Xenbase Image ID: 174766

Fig. 4. Mutation of the MgcRacGAP SxIP motif disrupts equatorial accumulation of the Centralspindlin complex. (A) Still images from live movies of NF stage 10-11 X. laevis embryos that had been co-injected with MO+MgcWT–3×GFP or MO+MgcSxNN–3×GFP (gray), a probe for MTs (2×mChe–EMTB, red) and a probe for DNA (mChe–H2B, red). (B) Still images from live movies of NF stage 10-11 X. laevis embryos that had been co-injected with MO+MgcWT or MO+MgcSxNN, along with MKLP1–3×GFP (gray), 2×mChe–EMTB (red) and mChe–H2B (red). (C) Schematic of contractile ring (CR) and polar cortex intensity quantification strategy. Quantification was performed at 25% cleavage furrow ingression. (D) Quantification of the Mgc–3×GFP intensity at the contractile ring. MO+MgcWT–3×GFP, 9 cells; MO+MgcSxNN–3×GFP, 10 cells. (E) Quantification of the Mgc–3×GFP intensity along the polar cortex of dividing cells. MO+MgcWT, 9 cells; MO+MgcSxNN, 10 cells. (F) Quantification of the MKLP1–3×GFP intensity at the contractile ring. MO+MgcWT, 8 cells; MO+MgcSxNN, 8 cells. (G) Quantification of the MKLP1–3×GFP intensity along the polar cortex of dividing cells. MO+MgcWT, 8 cells; MO+MgcSxNN, 8 cells. Scale bars: 10 µm. Mean±s.e.m. (unpaired Student’s t-test). All n values represent cumulative values from three or more independent experiments. Maximum projection images are shown and were used for quantification. Image published in: Breznau EB et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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