Xenbase Image ID: 174767

Fig. 5. The MgcRacGAP SxIP motif contributes to limiting and focusing RhoA-GTP and F-actin at the cell equator. (A) Still images from time-lapse movies of NF stage 10-11 X. laevis embryos that had been co-injected with MO+MgcWT or MO+MgcSxNN along with a probe for active RhoA (GFP–rGBD), a probe for F-actin (mChe–UtrCH) and a probe for DNA (mChe–H2B). Yellow arrowheads highlight RhoA-GTP accumulation along the equatorial cell cortex during early anaphase. (B) Same cells as in A showing the F-actin accumulation during early anaphase. (C) Schematic of the analysis approach for fitting a Gaussian curve to the intensity plot data to determine FWHM (breadth). The intensity of RhoA-GTP at the equatorial cortex just after anaphase onset and the intensity of F-actin accumulation during early anaphase (at 25% ingression), were measured. The FWHM is graphed as a percentage of cell width. (D) FWHM quantification of the breadth of RhoA-GTP accumulation at the equatorial cortex. MO+MgcWT=6 cells; MO+MgcSxNN=9 cells. (E) RhoA-GTP intensity quantification at the equatorial cortex. MO+MgcWT=6 cells; MO+MgcSxNN=9 cells. (F) FWHM quantification of F-actin contractile ring breadth. MO+MgcWT=8 cells; MO+MgcSxNN=6 cells. (G) F-actin contractile ring intensity quantification. MO+MgcWT=8 cells; MO+MgcSxNN=6 cells. Scale bars: 10 µm. Mean±s.e.m. (unpaired Student’s t-test). ns, not significant. All n values represent cumulative values from three or more independent experiments. Maximum projection images are shown and were used for quantification. Image published in: Breznau EB et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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