XB-IMG-174768
Xenbase Image ID: 174768
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Fig. 6. The MgcRacGAP SxIP motif is necessary for proper adherens junction structure. (A,C,E) Fixed maximum projection en face and side-view images of
mosaic NF stage 10-11 X. laevis embryos. The mosaic injection strategy allows for approximately half of the embryo to express the indicated knockdown and
rescue constructs along with GFP-membrane or mChe-membrane as a lineage tracer, while the other half of the embryo serves as an internal control and is not
injected. The dotted white line in the merged en face image represents the boundary between the internal control and injected region of mosaic embryos.
Antibodies against GFP and one of Mgc (A), β-catenin (C; β-cat) or E-cadherin (E; E-cad) were applied to the embryos after fixation. Side views show the
localization of the indicated protein along the apicobasal axis of cell–cell junctions (red arrowheads, internal control regions; green arrowheads, injected regions).
(A) Mgc (red) and GFP-membrane (green). (B) Quantification of the normalized Mgc intensity at bicellular junctions from the apical to basal surfaces. Control, 13
cells; MO+MgcWT, 14 cells; MO+MgcSxNN, 19 cells. (C) β-catenin (red) and GFP-membrane (green). (D) Quantification of the normalized β-catenin intensity
along the bicellular junctions from the apical to basal surfaces. Control, 17 cells; MO+MgcWT, 16 cells; MO+MgcSxNN, 19 cells. (E) E-cadherin (pseudo-colored
red) and mChe-membrane (pseudo-colored green). (F) Quantification of the normalized E-cadherin intensity along the bicellular junctions from the apical to basal
surfaces. Control, 19 cells; MO, 7 cells; MO+MgcWT, 15 cells; MO+MgcSxNN, 19 cells. Error bars represent s.e.m. (unpaired Student’s t-test). ns, not significant.
Scale bars: 20 µm. All n values represent cumulative values from three independent experiments. Image published in: Breznau EB et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View |