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Fig. S4. Expression analyses of hox genes by qPCR. [part3] Using the same data sets of qPCR as Fig. 3 or Fig. S3, the numbers of de novo synthesized transcripts were calculated. The data was analyzed using the Tukey-Kramer test and compared. Significant differences among data points are indicated in lowercase alphabets (for example, "b" represents significantly different from "a", while no significant difference was detected between the same alphabets). Two consecutive data points which show significant differences were connected and the intercept to the x-axis (time) was calculated as the onset of active transcription, except for hoxc3, where three points (10, 11.5, and 12.5 hpf) were used for the calculation (see Experimental Procedures). Image published in: Kondo M et al. (2019) © 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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