XB-IMG-174916
Xenbase Image ID: 174916
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Figure 3. DynAPs are MCC-specific and controlled by the motile ciliogenic transcriptional circuitry.(A) Membrane labeling with CAAX-RFP at the apical surface reveals a single MCC (cilia, dashed circle) surrounded by non-ciliated goblet cells. (a’) Projection of the cytoplasm of the same cells in A. GFP-Ktu is expressed throughout, but forms foci only in the MCC. (B) Membrane labeling at the apical surface reveals that expression of Mcidas converts all cells to MCCs. (b’) Projection of the cytoplasm of the same cells in B; GFP-Ktu forms foci in all cells upon expression of Mcidas.( C–D) Apical surface views of a control mucociliary epithelium (C) and one ectopic expressing Foxj1 (D). (c’–d’) Projection of the cytoplasm of the same cells in C and D. GFP-Dnai1 labels both of axonemes at the surface in C, D and DynaAPs in cytoplasm in c’d’. CAAX-BFP labels membranes; Dashed lines mark MCCs. Expression of Foxj1 induces solitary ectopic motile cilia (D) and ectopic DynAPs (d’).(E) Graph displaying number of cytoplasmic GFP-Ktu foci in wild-type MCCs and goblet cells as well as ciliogenesis-induced goblet cells. p < 2.2×10−16 for Foxj1 and p = 1.68×10−7 for Mcidas experiments by two-sample t-test. n = 29 (wild-type MCC), n = 130 (wild-type goblet cell), n = 122 (Foxj1-OE goblet cells), and n = 24 (Mcidas-HGR Goblet cells) across three embryos. Scale bars 10 µm. Image published in: Huizar RL et al. (2018) © 2018, Huizar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |