Xenbase Image ID: 174917

Figure 4. DynAPs display liquid-like behaviors.(A) Stills from time-lapse imaging of an individual DynAP labeled with GFP-Ktu undergoing fission (upper) and later coalescence (lower) (time in seconds). (B) Time-lapse images of GFP-Ktu recovery after photobleaching of partial (upper) or entire DynAPs (lower). Images are color-coded to highlight changes in pixel intensity (blue = low; red = high; green = intermediate. Dashed line marks the photobleaching area. (C) Kymograph of GFP-Ktu recovery after partial bleach, showing that recovery of the bleached area occurs from within the DynAP, rather than from the cytoplasm. Note, however, that FRAP of whole DynAPs indicates that rapid exchange also occurs between DynAPs and the cytoplasm (Panel B, lower; and see Table 2). (D) FRAP kinetics of GFP-Ktu after bleaching entire DynAPs. (E) FRAP kinetics of the trans-golgi protein GalT-GFP after bleaching golgi-derived vesicles. Image published in: Huizar RL et al. (2018) © 2018, Huizar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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