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Figure 6. DynAPs share molecular and physical properties with stress granules.(A) GFP-G3bp1 strongly co-localizes with DNAAFs in DynAPs (ovals), but also labels smaller foci that do not contain DNAAFs (boxes). (a1–a3) Higher magnification views of the bottom left corner of the MCC shown in panel A. (B) Quantification of co-localization relative to mCherry-Ktu (Dnaaf4 and Fus data from Figure 1 are recapitulated here for comparison). (C) FRAP kinetics of GFP-G3bp1 in Ktu-positive DynAPs (black) in MCCs and in Ktu-negative foci in neighboring goblet cells (pink). p < 0.0001 by one-way ANOVA and post-hoc Tukey-Kramer HSD test. n-values for each FRAP experiment can be found in Table 2. Image published in: Huizar RL et al. (2018) © 2018, Huizar et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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