Xenbase Image ID: 174928

Fig. 2. Ectopic Tbx2 suppresses mesendoderm induction. A: Tbx2 inhibits Activin-induced mesendoderm. RT-PCR analysis of animal cap explants dissected at late blastula stages and cultured until midgastrula stages. Activin (0.25 ng/ml) was added to stage 8 animal caps excised from uninjected embryos or from embryos injected with tbx2 RNA into the animal pole of both blastomeres at the two-cell stage. B: Inhibition of FGF-mediated mesoderm induction by Tbx2. RT-PCR analysis of animal cap explants dissected at late blastula stages and cultured until midgastrula stages. Basic FGF (10 ng/ml) was added to stage 8 animal caps excised from uninjected embryos or from embryos injected with tbx2 RNA, as listed. Unless otherwise noted, 1 ng of tbx2 RNA was used in this and in subsequent experiments. Image published in: Teegala S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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