Xenbase Image ID: 174930

Fig. 4. Tbx2 knockdown leads to ectopic expression of mesendodermal marker genes. A: Tbx2 morpholino (Tbx2MO) blocks translation of tbx2 RNA in vitro. MObs-Tbx2 is a tbx2 construct that contains the Tbx2MO-binding site. B: Mesendodermal markers are up-regulated in ectodermal explants derived from Tbx2 morpholino-injected embryos. RT-PCR analysis of animal cap explants dissected at late blastula stages and harvested at mid-gastrula stages; embryos were injected with Tbx2 morpholino (80 ng) at early cleavage stages. For the rescue experiment, 80 ng of Tbx2 morpholino and 1 ng of tbx2 RNA were coinjected at early cleavage stages. C: Injection of a control (80 ng), “scrambled” morpholino does not lead to extra-ectodermal gene expression, while injection of Tbx2 morpholino (80 ng) results in ectopic expression of both mesodermal and endodermal markers. Image published in: Teegala S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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