XB-IMG-174932
Xenbase Image ID: 174932
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Fig. 6. Tbx2 functions as a repressor during germ layer differentiation.
A: Expression of Tbx2-DBD-EnR inhibits mesendoderm induction by
Activin (left panel); expression of Tbx2-DBD-Vp16 does not inhibit
Activin-mediated mesendoderm induction (right panel). RT-PCR analysis
of animal caps dissected at late blastula stages and cultured until midgastrula
stages. tbx2-DBD-EnR or tbx2 DBD-VP16 RNA (1 ng) was
injected at early cleavage stages, as indicated. Activin (0.5 ng/ml) was
added to stage 8 animal caps, as indicated. B: Expression of Tbx2 DBDVP16
fails to inhibit BMP target gene expression (left panel), and stimulates
expression of mesodermal and endodermal marker genes in the
absence of exogenous growth factors (left and right panels). RT-PCR
analysis of animal caps dissected at late blastula stages and cultured
until mid-gastrula stages. tbx2-DBD-VP16 RNA (1 ng) was injected at
early cleavage stages, as indicated. The “-RT” lane contains all reagents
except reverse transcriptase and was used as a negative control. Image published in: Teegala S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |