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Fig 5. Plot of expected heterozygosity (HE) against generation number (t). The curved dotted strain indicates theoretical reduction of heterozygosity via single brother-sister matings for every generation, calculated from the following equation: , where H0 is the heterozygosity of the founder colony, and Ne is effective population size (i.e., number of individuals that contribute to breeding). HE of F0 of N (Gurdon Inst.) (0.509) and 2 are used for H0 and Ne, respectively. Open rectangles indicate mean HE estimated from individual based simulation using VORTEX version 10 [43]. During simulation, allele frequencies of N (Gurdon Inst.) were used as initial allele frequency, and single brother-sister mating was conducted in every generation. The simulation was repeated one hundred times. Bar indicates standard deviation. Image published in: Igawa T et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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