XB-IMG-174977
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Figure 3
Expression of neural tissue-related and synapse-related genes in the brain of NF stage 61 wild-type and TR-knockout tadpoles. Total cellular RNA was extracted from brains of NF stage 61 wild-type, TR-knockout, and TR-knockout tadpoles, and qRTPCR was conducted using gene-specific primers (Supporting Information Table S2). The relative mRNA expression level of the indicated gene to EF1 mRNA is presented. Data are expressed as mean SD. Biological replicate numbers of wild-type, TR-knockout, and TR-knockout brain samples are five, five, and four, respectively. Statistical significance by comparison of TR-knockout tadpoles with wild-type and TR-knockout tadpoles were assessed using the Tukey's test. *p < 0.05, **p < 0.01 Image published in: Yaoita Y and Nakajima K (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons.
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