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Supplementary Figure 1. Physical interaction of Smad-1 and Xbra uniquely regulates ventx1.1 transcription activation, but not that of Xvent2, in the Xvent family. We co-injected the Xvent2 promoter region construct with Xbra and Smad-1, in combination or separately, at the 1-cell stage and dissected the animal cap of injected embryos at stage 8. Dissected animal caps were also harvested in L-15 culture media until stage 11, followed by RT-PCR and reporter gene assay of the samples. (a) Xvent2 (-1031) promoter region injected with Xbra and Smad-1, in combination or separately. (b) Embryos were co-injected with Xbra and Smad-1, separately or in combination to perform the RT-PCR at the stage 11. All relative promoter activity experiments were performed in triplicate. All relative promoter activity data are shown as mean ± SE. Image published in: Kumar S et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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