Xenbase Image ID: 175191

Figure. S4. Distribution of endogenous Notch 1 and β-catenin proteins in Xenopus mid blastula (internal views). Internal views of right (A-D’’) and left (F-O´) halves of embryos that were cut at s8 (as shown in the left column, turquoise dotted boxes) and processed for whole-mount IF. The embryonic early axes were predicted according to the original pigmentation. (A,F,F’,K,K’) Bright field views. (B-B'',G,G’,L,L’) Notch1 IF. (C-C'',H,H’,M,M’) Total β-catenin IF. (D-D'',I,I’,N,N’) Merged images of Notch1 and total β-catenin IF. (E-E'',J,J’,O,O’) Nuclear Hoechst staining. The vertical yellow dotted lines in (B-E) indicate the photocomposition of images taken at two slightly different focal planes to keep nuclei in focus in the dorsal and ventral region of the embryo. (B'-E') Higher magnification of the ventral region of the images shown in B-E, corresponding to the area indicated by the left red box in E. (B''-E'') Higher magnification of the dorsal region of the images shown in B-E, corresponding to the area indicated by the right red box in E. (F-J) and (K-O) Ventral and dorsal regions, respectively, of the same embryo. (F’-J’) and (K’-O’) Higher magnifications of F-J and K-O, respectively, corresponding to the yellow boxed area in F and K. Notch1 protein is more enriched in the ventral (white asterisks) than in the dorsal region (yellow asterisks) whereas the opposite occurs with nuclear β-catenin (white arrows). These patterns were consistently found both in right and left halves at this stage in embryos from different batches (94%, n=18, 3 batches of embryos; see Table S2). Interestingly, amongst the ventral cells, we could distinguish Notch1+ nuclei in several cells (G’,I’,J’, light blue arrows) while others did not appear to have nuclear Notch1 accumulation (G’,I’,J’, pink arrows). Bl, blastocoel cavity; d, dorsal; v, ventral; l, left; r, right; an, animal; veg, vegetal. Image published in: Castro Colabianchi AM et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue notch1.L notch, notch-1, x-notch-1, xnotch, xnotch1, xotch X. laevis Throughout NF stage 8 animal cap nucleus ventral marginal zone ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 8 animal cap blastocoel floor vegetal endoderm marginal zone nucleus Image source: Published Larger Image Printer Friendly View

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