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Fig. 4. Reduced number of terminally differentiated cardiomyocytes upon loss of Dkk1. A. Sections of embryos are shown stained for cardiac troponin T (red) to label cardiomyocytes and phosho-histone H3 (PH3, green) lo mark proliferating cells and counterstained with DAPI (blue) to highlight nuclei at the stages indicated. Yellow arrowheads indicate proliferating cardiomyocytes positive for cardiac troponin and phosho-histone H3. B. Cardiac troponin T positive cardiomyocytes were quantified on continuous serial sections of stained embryos. Embryos bilaterally injected with Dkk1 MO showed reduced number of cardiomyocytes at the stages indicated. C. The mitotic index (MI) as defined by the percentage of phosho-histone H3 positive cardiomyocytes is not affected upon Dkk1 MO injection. N = number of independent embryos counted. *p < 0.05, **p < 0.01, with Mann-Whitney rank sum test. Scale bar, 100 μm. Image published in: Guo Y et al. (2019) © 2019 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Gene Synonyms Species Stage(s) Tissue tnnt2.L CT3, LOC446916 X. laevis Throughout NF stage 33 and 34 cardiac myocyte tnnt2.L CT3, LOC446916 X. laevis Throughout NF stage 37 and 38 cardiac myocyte tnnt2.L CT3, LOC446916 X. laevis Throughout NF stage 42 cardiac myocyte h3-3a.L h3.3, h3.3a, h3f3a X. laevis Throughout NF stage 33 and 34 cardiac myocyte mitotic cell h3-3a.L h3.3, h3.3a, h3f3a X. laevis Throughout NF stage 37 and 38 mitotic cell cardiac myocyte h3-3a.L h3.3, h3.3a, h3f3a X. laevis Throughout NF stage 42 mitotic cell cardiac myocyte Image source: Published Larger Image Printer Friendly View

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