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Fig. 6. The expression of differentiation cardiac markers is delayed upon loss of Dkk1. Heart-enriched explants as illustrated on the top (marked in pink) were isolated from stage 28 and 34 embryos bilaterally injected with either Control MO or Dkk1 MO. Expression of cardiac marker genes in these explants was analyzed by real-time RT-PCR and presented as relative expression to gapdh. Median values are given. The whiskers indicate the maximum and minimum values. n = number of independent experiments (n = 5–10). *p < 0.05, **p < 0.01, n.s. not significant with Mann-Whitney rank sum test. Image published in: Guo Y et al. (2019) © 2019 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |