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Supplement Fig. 4 Mesoderm formation and cardiac specification are not dependent on Dkk1.
A. Expression of mesoderm marker tbxt is not affected upon Dkk1 knockdown. tbxt expression was analyzed at stage 10 embryos injected with Dkk1 MO or Control MO at stage 4. Red dotted circles indicate the location of the cardiac progenitor cell population induced at the onset of gastrulation. Quantification is shown in B. C. Expression of early cardiac markers is not altered upon Dkk1 depletion. Gene expression was analyzed at stage 13 embryos injected with Dkk1 MO or Control MO at stage 4. Quantification is shown in D. Mean values with standard errors are given. n = number of independent experiments, N = total number of embryos analyzed.
Image published in: Guo Y et al. (2019) © 2019 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license
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