XB-IMG-175287
Xenbase Image ID: 175287
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FIGURE 2. Knockdown of Dnmbp results in reduced kidney tubulogenesis. (A) Western blot showing the efficiency of two different MOs targeting Dnmbp. Single cell embryos were injected with 40ng of Dnmbp MO 1, Dnmbp MO 2 or Standard MO. One embryo equivalent per lane was loaded on to the SDS-PAGE gel. (B) Schematic of the Xenopus pronephros showing the proximal, distal and connecting tubule regions. (C) Unilateral injection of 20ng Dnmbp MO 1 into blastomere V2 at the 8-cell stage leads to defects in kidney tubulogenesis in comparison to embryos injected with Standard MO. Antibody 3G8 used to label the lumen of the proximal tubule, antibody 4A6 used to label the distal and connecting tubules. memRFP used as an injection tracer. White bar indicates 200 μm.∗ indicates injected side of embryo. (D) Knockdown of Dnmbp leads to reduced expression of differentiated kidney tubule markers in comparison to embryos injected with Standard MO. n = number of embryos across 3 replications. Error bars represent Standard error. ∗Significantly different from control, p < 0.05. Image published in: DeLay BD et al. (2019) Copyright © 2019 DeLay, Baldwin and Miller. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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