Xenbase Image ID: 175289

FIGURE 4. Dnmbp knockdown alters ciliogenesis. (A) Unilateral injection of 20ng Dnmbp MO 1 into blastomere V2 at the 8-cell stage causes altered primary ciliogenesis in comparison to embryos injected with Standard MO. Longer primary cilia are visible in the proximal tubules of the Dnmbp knockdown embryos (“more prominent cilia” phenotype). Acetylated tubulin antibody used to label cilia and nerves and lectin used to label the proximal tubule lumen. Images are stitched from 6 tiles. White bar is 50 μm, yellow bar is 25 μm. White arrowhead indicates nephrostome. White box indicates region represented in the enlarged merge panel. (B) Knockdown of Dnmbp leads to altered primary cilia development compared to embryos injected with Standard MO. n = number of embryos across 3 replications. Error bars represent Standard error. ∗Significantly different from control, p < 0.05. Image published in: DeLay BD et al. (2019) Copyright © 2019 DeLay, Baldwin and Miller. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tuba1a.L X. laevis Throughout NF stage 29 and 30 pronephric kidney ciliated cell cilium Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dnmbp MO + NF40-41 (immunohistochemistry) tuba4a Fig. 4 43, r4 Anatomical Phenotype abnormal multiciliated cell abnormal pronephric nephrostome morphology abnormal pronephric tubule morphology abnormally increased number of multiciliated cell Larger Image Printer Friendly View

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