Xenbase Image ID: 175294

FIGURE 9. dnmbp CRISPR knockout does not alter expression of early markers of kidney development, however late marker expression is altered by in situ analysis. Embryos injected unilaterally at the 8-cell stage (blastomere V2) to target the kidney and reared to stage 29–30 for lhx1 expression assessment and stage 40–41 for atp1a1 expression assessment. Representative embryo phenotypes shown. Scale bar indicates 500 μm. n = number of embryos across 2 replications. Error bars represent Standard error. ∗Significantly different from control, p < 0.05. Image published in: DeLay BD et al. (2019) Copyright © 2019 DeLay, Baldwin and Miller. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 28 pronephric mesenchyme atp1a1.L atp1a1-a, atp1a1-b, K-ATPase, Na+/K+ ATPase, Na+K+ ATPase, Na+K+ATPase X. laevis Throughout NF stage 28 pronephric nephron pronephric tubule proximal tubule distal tubule Image source: Published Experiment + Assay Source Phenotypes and Disease Xla wt + dnmbp CRSPR + NF40-41 (in situ hybridization) Fig. 9 Anatomical Phenotype abnormal pronephric tubule morphology Expression Phenotype decreased amount atp1a1.L expression in distal tubule decreased amount atp1a1.L expression in proximal tubule Larger Image Printer Friendly View

Return to previous page