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FIGURE 10. Dnmbp knockdown alters expression of late markers of kidney development, as assessed by in situ analysis. Embryos injected unilaterally at the 8-cell stage (blastomere V2) to target the kidney and reared to stage 40–41 for slc5a1, clcnkb, and atp1a1 expression assessment or stage 36–37 for nphs1 expression assessment. Representative embryo phenotypes shown. Scale bar indicates 500 μm. n = number of embryos across 2 (slc5a1, clcnkb, nphs1) or 3 replications (atp1a1). Error bars represent Standard error. ∗Significantly different from control, p < 0.05. Image published in: DeLay BD et al. (2019) Copyright © 2019 DeLay, Baldwin and Miller. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue slc5a1.2.L D22S675, sglt-1, sglt-1k, sglt1 X. laevis Sometime during NF stage 40 to NF stage 41 glomus pronephric kidney pronephric nephrostome clcnkb.L ClC-k, clc-kb, clckb, clcnka, clk-k2, xClC-k X. laevis Sometime during NF stage 40 to NF stage 41 pronephric kidney pronephric tubule nphs1.L nephrin X. laevis Throughout NF stage 40 to NF stage 41 pronephric kidney atp1a1.L atp1a1-a, atp1a1-b, K-ATPase, Na+/K+ ATPase, Na+K+ ATPase, Na+K+ATPase X. laevis Sometime during NF stage 40 to NF stage 41 pronephric kidney pronephric nephron pronephric tubule proximal tubule distal tubule pronephric duct Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dnmbp MO + NF35/36-37/38 (in situ hybridization) Fig. 10 Expression Phenotype absent nphs1.L expression in pronephric tubule Xla Wt + dnmbp MO + NF40-41 (in situ hybridization) Fig. 10 Expression Phenotype decreased amount atp1a1.L expression in distal tubule decreased amount atp1a1.L expression in proximal tubule decreased amount clcnkb.L expression in proximal tubule decreased amount slc5a10.L expression in proximal tubule Larger Image Printer Friendly View

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