Xenbase Image ID: 175319

Supplementary Figure 2. Cilia number in the GRP of fgfr4 CRISPR-1 F0 embryos. (A) An example of how GRPs were outlined in order to count cilia and measure GRP area. (B,C) The total number of cilia per GRP is reduced in fgfr4 CRISPR embryos (B), however when the cilia numbers are normalized to total GRP area, no difference is visible between control and CRISPR embryos (C). Scale bar = 40 μm. Image published in: Sempou E et al. (2018) Copyright © 2018 Sempou, Lakhani, Amalraj and Khokha. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue actb beta actin, beta-actin, g-actin X. tropicalis Throughout NF stage 17 left-right organizer gastrocoel roof plate tuba4b alpha tubulin, alpha-tubulin, tuba4, tuba4a X. tropicalis Throughout NF stage 17 left-right organizer Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + fgfr4 CRISPR + NF17 (immunohistochemistry) Fig. S 2 A, B Anatomical Phenotype abnormal gastrocoel roof plate morphology abnormal left-right organizer morphology abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Expression Phenotype decreased amount tuba4b expression in left-right organizer Larger Image Printer Friendly View

Return to previous page