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FIGURE 4. Differential effect of Wnt/beta catenin signaling activation on cardiac and liver cell fate. (A) Animal caps co-injected with Gata4 mRNA and Wnt8-expressing DNA show marked reduction of myl7 RNA levels and negligible effect on nr1h5 expression. (B) qPCR analysis confirms the findings of gel RT-PCR for myl7 and nr1h5 and in addition shows the reduction of hhex expression in Gata4 + Wnt8 samples. (C) AC/AE conjugates were treated with LiCl at indicated stages. Treatment at st. 21 blocked cardiac differentiation but had a lesser effect on liver specification. (D) Cell autonomous activation of Wnt/β-catenin signaling in LEF-β-GR mRNA-injected AE was achieved by adding dexamethasone (dex) to AC/AE conjugates at indicated stages. No effect on either heart or liver marker expression was observed. (E) In contrast, activation of LEF-β-GR in the AC part of the conjugates greatly reduced myh6 expression without affecting nr1h5. Explants were collected for RT-PCR analysis when sibling embryo controls reached st. 34. Image published in: Haworth K et al. (2019) Copyright © 2019 Haworth, Samuel, Black, Kirilenko and Latinkic. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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