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FIGURE 7. FGF signaling is not required for liver specification by Gata4 but is essential in AC/AE explants. (A) Gata4 and XFD (dominant-negative FGFR1) mRNA co-expression in animal pole explants has no effect on nr1h5 and myl7 expression. (B) qPCR analysis confirms these findings and extends them by showing no significant effect on hhex and foxa2. (C) Treatment of AC/AE with FGFR inhibitor SU5402 (50 μM) has stage-specific effect. Immediately upon AC/AE conjugate formation, SU5402 inhibits cardiac and liver gene expression. Treatment from st. 13 until the end of incubation at st. 34 has no effect on cardiogenesis but inhibits liver specification. Shorter time windows of treatment are largely without effect. Staging is according to the stage of AE (st. 10 at the time of conjugation). C-Control explants treated with 50 μM DMSO. (D) SU5402 and XFD are effective inhibitors of early mesodermal marker and FGF target gene tbxt. CE-control (untreated) sibling embryos. RT-PCR analyses were performed on st. 34 explants and sibling embryo controls. Image published in: Haworth K et al. (2019) Copyright © 2019 Haworth, Samuel, Black, Kirilenko and Latinkic. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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