Xenbase Image ID: 175344

Supplemental Fig. S1. ELMO1 localizes to the ciliary axonemes in MCCs and validation of antibodies used for human respiratory epithelial cells and MDCK studies (A-D) Human respiratory epithelial cells from controls were double-labelled with antibodies directed against ELMO1 (magenta) and acetylated Tubulin (green). ELMO1 localizes along the ciliary axonemes (white arrow) and the basal bodies (white arrowhead). The nucleus is stained with Hoechst33342 (blue). Immunoblots of human respiratory epithelial cell lysates and MDCK lysates validates the utilized ELMO1 antibody with the appropriate double bands according to the manufacturer (E,G), Ezrin (F) and DOCK1 (H) antibodies. Scale bar, 10 μm (D). Image published in: Epting D et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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