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Supplemental Fig. S2. ELMO1 and DOCK1 localize to the basal body of primary cilia (A) MDCKs were grown to ciliated stage and stained against ELMO1 (green) and the cilia marker Tubulin (detyrosinated) (magenta). The merged image illustrates distinct ELMO1 staining at the base of the cilium. (B) DOCK1 (green) was co-stained with Tubulin (detyrosinated) (magenta). The merged image illustrates distinct DOCK1 staining at the base of the cilium. (C) ELMO1 (green) was co-stained with DOCK1 (magenta) and Cep164 (blue), a marker for the mother centriole. Co-localization of ELMO1 and DOCK1 at the mother centriole is indicated by yellow pixels. Scale bars, 10 μm (A,B), 1 μm (C).

Image published in: Epting D et al. (2015)

Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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