Xenbase Image ID: 175361

Figure S1. Schematic for generating transcribed PCR products containing poly (A) tails (TPATs) for injecting Xenopus embryos. The Harvard Institute of Proteomics FLEXGene human kinase cDNA collection was used to amplify kinase coding regions. The 5’ primer was designed to introduce a T7 promoter to the 5’ end of the amplified PCR product. To increase the stability of the kinase mRNAs upon their injection into Xenopus embryos, a poly(A) sequence from the pCS2+ plasmid was also amplified by PCR. The products from the kinase and poly(A) reactions were then stitched together in a third PCR reaction to generate kinase cDNAs containing a 5’ T7 promoter and a 3’ poly (A) tail. These DNAs were subsequently used for in vitro transcription reactions to generate TPATs. Image published in: Neitzel LR et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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