XB-IMG-175362
Xenbase Image ID: 175362
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Figure S2. TPAT mRNAs demonstrate robust expression in Xenopus embryos. (A) Uninjected Xenopus embryos. (B) Xenopus embryo injected with GFP TPAT without the poly(A) tail demonstrates no observable expression of GFP compared to background. Xenopus embryos injected with GFP TPAT without (C) or with (D) purification of final PCR products show robust GFP expression. Each dorsal blastomere of a 2-cell embryo was injected with 1 ng mRNA and allowed to develop to neurula stages (20-21) prior to imaging. Results are aggregates of n≥3 replicates. Image published in: Neitzel LR et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |