Xenbase Image ID: 175367

Figure S7. The phenotype cause by disrupting UDP-GlcNAc salvage pathway components in Xenopus embryos is rescued by injecting downstream sugar intermediates. (A) Co-injecting GlcNAc-6-P, GlcNAc-1-P, or UDP-GlcNAc rescues the anteriorization phenotype caused by NagkT128M mRNA injection. (B) Co-injecting GlcNAc-6-P, GlcNAc-1-P, or UDP-GlcNAc rescues the anteriorization phenotype caused by 3-OMe-GlcNAc injection. (C) Co-injecting GlcNAc-6-P, GlcNAc-1-P, or UDP-GlcNAc rescues the anteriorization phenotype caused by Nagk MO injection. (D) Co-injecting GlcNAc-1-P or UDP-GlcNAc rescues the anteriorization phenotype caused by Pgm3 MO injection. (E) Co-injecting UDP-GlcNAc rescues the anteriorization phenotype caused by Uap1 MO injections. Absolute numbers are indicated above bars. Results are aggregates of n≥3 replicates. Each dorsal blastomere was injected with 1.5 ng mRNA, 1 pg MO, and/or 125 pmol sugar. Significance was calculated using Fisher’s exact test with Bonferroni correction. (A-C) *ρ< 0.0170, **ρ< 0.00334, ***ρ< 0.000334. (D) *ρ< 0.0253. (E) *ρ< 0.05. Image published in: Neitzel LR et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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