Xenbase Image ID: 175368

Figure S8. The phenotype caused by injecting UDP-GlcNAc salvage pathway sugars in Xenopus embryos is rescued by downregulating salvage pathway enzymes. (A) Co-injecting NagkT128M mRNA or 3-OMe-GlcNAc rescued the posteriorization phenotype caused by GlcNAc injection. (B) Co-injecting Nagk MO, Pgm3 MO, Uap1 MO, or Dpagt1 MO rescued the posteriorization phenotype caused by GlcNAc injection. (C) Co-injecting Pgm3 MO, Uap1 MO, or Dpagt1 MO rescued the posteriorization phenotype caused by GlcNAc-6-P injection. (D) Co-injecting Uap1 MO or Dpagt1 MO rescued the posteriorization phenotype caused by GlcNAc-1-P injections. (E) Co-injecting Dpagt1 MO rescued the posteriorization phenotype caused by UDP-GlcNAc injections. Absolute numbers are indicated above bars. Each dorsal blastomere was injected or co-injected with 1.5 ng mRNA, 1 pg MO, and/or 125 pmol sugar. Significance was calculated using Fisher’s exact test with Bonferroni correction. (A and D) *ρ< 0.0253, **ρ< 0.00501. (B) *ρ< 0.0127, **ρ< 0.00251. (C) *ρ< 0.0170, ***ρ< 0.00334. (E) *ρ< 0.05. Image published in: Neitzel LR et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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