Xenbase Image ID: 175371

Figure S13. Expression of Chordin in Xenopus ectodermal explants suggests the UDP-GlcNAc salvage pathway works at the level of the ligand and/or receptor. (A) (Left) Activation of the Wnt pathway by injection of Xwnt8 mRNA (as indicated by expression of the Wnt target gene, Chordin) is inhibited when co-injected with MOs against salvage pathway enzymes. (Right) Conversely, co-injection with mRNA encoding salvage pathway enzymes enhances Chordin expression. Similarly, induction of Chordin by Wnt8-Fz5 (B) or Lrp6 (C) was inhibited or enhanced upon co-injection with MOs or mRNA for salvage pathway enzymes, respectively. (D) In contrast, activation of the Wnt pathway by Dsh was unaffected by inhibition (Left) or overexpression (Right) of the salvage pathway enzymes. Data shown is representative of n≥3 biological replicates using n=3 technical replicates. Each replicate was a pool of n=5 animal caps. Each dorsal blastomere was injected with 1 ng mRNA and/or 1 pg MO. Expression was normalized to Odc. Graphs display fold change calculated using the 2-ΔΔCt method. Significance was calculated using T-tests with equal variance and Bonferroni correction on the ΔCt values. *ρ< 0.0127, **ρ<0.00251, ***ρ<0.000251. Image published in: Neitzel LR et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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