XB-IMG-175395
Xenbase Image ID: 175395
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Supplementary Figure 1: of Expression patterns of NC genes in embryos affected by leflunomide (A) The in situ hybridisation data was quantified based on whether embryos displayed an expansion, wild type, partial loss or loss of expression phenotype. This is displayed as a percentage of embryos displaying these phenotypes after DMSO treatment (Top panel) for c-Myc (n=106), Sox10 (n=49), Slug (n=54), FoxD3 (n=47), Sox9 (n=39), Zic1 (n=40), Zic3 (n=45), Pax3 (n=43), Sox2 (n=43) and also 60μM leflunomide treatment (bottom panel) for c-Myc (n=108), Sox10 (n=56), Slug (n=62), FoxD3 (n=50),Sox9 (n=47), Zic1 (n=37), Zic3 (n=43), Pax3 (n=34), Sox2 (n=39). (B) qRT- PCR showing level of mRNA expression after 60μM leflunomide treatment compared to DMSO treated whole embryos. Left panel shows the level of expression of Zic1, Zic3, Pax3, Sox2 and a negative reverse transcription (RT) control. No significant change in expression was seen for any of these genes ns = not significant. Right panel shows the level of expression of c-Myc, Sox10, Slug, Sox9 and a negative RT control. All of these show a significant decrease in expression level. *=p<0.05, **=p<0.0000001 and ***=p<0.0000000001. (C) RNA-Seq Graph showing the percentage of genes downregulated in the neural crest animal caps sorted by category. NC = neural crest specific genes, HSP = heat shock proteins, MMP’s = matrix metalloproteinases. Image published in: Hatch VL et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |