Xenbase Image ID: 175399

Supplemental Figure 5: Knockdown of pTEFb components CDK9a or CyclinT1 does not affect expression of neural markers. All embryos were injected in one cell of a two cell stage embryo with either a control morpholino CoMo, 100ng CDK9b morpholino or 60ng CyclinT1 morpholino. 300pg of Lac-Z cRNA was co-injected with morpholinos and β-gal activity was detected using a red gal stain to monitor lineage tracing. in situ hybridisation was then carried out on these embryos for neural markers. Como injected Hbox6 n=29, Engrailed n=31, Krox20 n=31, Otx2 n=29, Ncam n=26, N-tubulin n=32. Cdk9a mo injected Hbox6 n=20, Engrailed n=30, Krox20 n=31, Otx2 n=25, Ncam n=26, N-tubulin n=35. CyclinT1 mo injected Hbox6 n=27, Engrailed n=21, Krox20 n=33, Otx2 n=28, Ncam n=26, N-tubulin n=27. DMSO treated Hbox6 n=18, Engrailed n=22, Krox20 n=34, Otx2 n=31, Ncam n=19, N-tubulin n=20. Leflunomide treated Hbox6 n=30, Engrailed n=28, Krox20 n=34, Otx2 n=31, Ncam n=31, N-tubulin n=32. Image published in: Hatch VL et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 13 to NF stage 21 presumptive rhombomere otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 13 to NF stage 21 anterior placodal area neuroectoderm ncam1.L n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 13 to NF stage 21 neural plate tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 13 to NF stage 21 Rohon-Beard neuron anterior placodal area neural plate Image source: Published Larger Image Printer Friendly View

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