Xenbase Image ID: 175400

Supplemental Figure 6: c-Myc can rescue the effect of Leflunomide (A) Embryos were injected in one blastomere of a two cell stage embryo with c-Myc RNA. Embryos were then treated from stage 8 with 60uM Leflunomide. Embryos then underwent Sox10 in situ. Treating with leflunomide on its own leads to a loss of Sox10 expression. Injection of c-Myc into one blastomere leads to a partial rescue of Sox10 expression. (B) Graph showing the percentage of embryos displaying the wildtype pattern, partial loss or loss of Sox10 expression. Treatment with DMSO alone (n=30), 60μM leflunomide (n=41) and 60μM Leflunomide plus injection of 500pg of c-Myc RNA into one blastomere (n=35) Image published in: Hatch VL et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox10.L X. laevis Sometime during NF stage 15 to NF stage 16 neural plate border neural crest Image source: Published Larger Image Printer Friendly View

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