Xenbase Image ID: 175401

Supplemental Figure 7: Knockdown of P-TEFb components CDK9.S and CYCLINT1 does not affect Snail1 expression. All embryos were injected in one cell of a two cell stage embryo with either a control morpholino CoMo, 40ng CDK9a morpholino or 60ng of CyclinT1 morpholino. 300pg of Lac-Z cRNA was co-injected with morpholinos and β-gal activity was detected using an X-gal stain to monitor lineage tracing. in situ hybridisation was then carried out with a Snail1 probe. Note the Snail 1 expression on the injected side with the Cdk9 and CyclineT1 morpholinos looks unchanged or very slightly increased. Embryos are shown as dorsal views with anterior to the top. Image published in: Hatch VL et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai1.L LOC108702592, sna, snail, snail1, xSna, Xsnail X. laevis Throughout NF stage 13 to NF stage 21 neural plate border neural crest Image source: Published Larger Image Printer Friendly View

Return to previous page