Xenbase Image ID: 175410

Figure 4. Cardiac progenitor genes are upregulated at 3 days post‐light activation. (a) KillerRed‐expressing tadpole hearts exposed to light show a significant increase in expression of Gata4 relative to uninjected controls at 3‐days post‐light exposure (*p < 0.05; Kruskal–Wallis test). Changes in Gata4 expression were measured relative to Eef1a expression as an endogenous control. Error bars indicate SEM of dCt values. n = 10 tadpoles per replicate, N = 6 replicates. (b) KillerRed‐expressing tadpole hearts exposed to light show a significant increase in expression of Nkx2.5 relative to uninjected controls at 3 days post‐light exposure (**p < 0.01; Kruskal–Wallis test). Changes in Nkx2.5 expression were measured relative to Eef1a expression as an endogenous control. Error bars indicate SEM of dCt values. n = 10 tadpoles per replicate, N = 5 replicates. (c) In situ hybridization for Gata4 and Nkx2.5 shows that there is no change in which tissues these genes are expressed in following light activation of KillerRed‐expressing tadpole hearts. Both genes are expressed, among other tissues, in the myocardium of the ventricle (blue arrow), the myocardium of the outflow track (red arrow), and the inner lining (endocardium or apical myocardium) of the atrium (green arrow). Representative images from 3 days post‐light activation are shown here. Scale bar = 100 μm. Section thickness = 10 μm Image published in: Jewhurst K and McLaughlin KA (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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