XB-IMG-175421
Xenbase Image ID: 175421
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Figure 1. Prdm12 Expression in Dorsal Root Ganglia( A–W) Transverse DRG sections of embryonic or adult mice as indicated, double immunostained with Prdm12 (purple) and the indicated markers (green). (A) Immunostainings of Prdm12 and the neural crest transcription factor Pax3 at E11.(B–F) Immunostainings of Prdm12 and the pan-neuronal transcription factor Islet1 at E9.5 (B), E10.5(C), E12.5 (D), or E14.5 (E). The proportion ofPrdm12+, Islet1+, or Prdm12+/Islet1+ cells de-tected at the different analyzed stages is shown in (F)(mean±SD; n = 2 [E10.5], 3 [E9.5, E12.5, and E14.5],or 5 [E11.5]) embryos.(G) Immunostaining of Prdm12 and a Sox10 reporterconstruct enabling the vizualisation of Sox10+sat-ellite cells at E14.5. The inset shows a higher magnification view of an area with DAPI counter-staining (blue), and the arrowhead points to a putative Sox10+satellite cell surrounding a Prdm12+cell. (H, I, P, and S) Immunostainings of Prdm12 with the neurotrophic receptor TrkA at E12.5 (H), E14.5 (I), E18.5 (P), or in adult DRG (S).(J, K, and V) Immunostainings of Prdm12 with theneurotrophic receptor TrkB at E12.5 (J), E14.5 (K), orin adult DRG (V).(L, M, and W) Immunostainings of Prdm12 with theneurotrophic receptor TrkC at E12.5 (L), E14.5 (M), or in adult DRG (W). (N–O) Immunostainings of Prdm12 with the transcription factor Runx3 at E12.5 (N) or E14.5 (O). (Q–T) Immunostainings of Prdm12 with the nociceptor-specific ion channel Nav1.8 at E18.5 (Q) or in adult DRG (T). (R–U) Immunostainings of Prdm12 with the transcription factor Runx1 at E18.5 (R) or in adultDRG (U).In (B), (C), (H), (I), and (N), the insets are highermagnification views of a selected area demon-strating the extensive overlap of the Prdm12 stainingwith the second marker. DRGs are delineated withdashed lines. Scale bars in main pictures, 100mm; scale bars in magnified pictures, 25mm. Image published in: Desiderio S et al. (2019) Copyright © 2019. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |