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Figure 2. Prdm12 Is Expressed in Precursors of Both Ngn1- andNgn2-Dependent Neurogenic WavesTransverse sections of DRG of embryos with the indicated genotype, doubleimmunostained at the indicated stage with Prdm12 (purple) and the indicatedmarkers (green).(A–C) Comparison of the expression of Prdm12 with that of Ngn2- and Ngn1-expressing cells or with boundary cap-derived cells. Yellow arrowheads pointto Prdm12+precursors co-expressing the second analyzed marker (Ngn2 in A,GFP in B, and tdTomato in C).(D–I) Double immunostaining of Prdm12 and TrkA (D and G), TrkB (E and H), orTrkC (F and I) in DRG of E15.5 WT andNgn1GFP/GFPknockout embryos. Notethe severe loss of Prdm12+and TrkA+cells inNgn1GFP/GFPcompared to WTand that the few remaining TrkA+cells also express Prdm12 (D and G).(J) Transverse sections of WT and Ngn1 GFP/GFPE11.5 embryos hybridized with Ngn1 or Prdm12 antisense probes.Prdm12 expression at that stage appears unaffected by the absence of Ngn1. DRG and spinal cord are delineated with dashed lines. Scale bars, 100mm. Image published in: Desiderio S et al. (2019) Copyright © 2019. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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