XB-IMG-175423
Xenbase Image ID: 175423
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Figure 3. Somatosensory Ganglia Hypoplasia inPrdm12KO Embryos Is Due to the Selective Loss of Nociceptors by Apoptosis(A) Lateral views of X-gal-stained E14.5 controlPrdm12LacZ/+andPrdm12LacZ/LacZembryos (top). High-magnification views of the head (middle) and dorsal viewof the body (bottom) are shown. Note the reduced size of TG and DRG and the loss of SJG (indicated by white arrowheads) in thePrdm12LacZ/LacZembryos. Scalebars, 1,000mm.(B) Transverse sections of the SJG of E13.5 wild-type (WT) andPrdm12KO embryos double immunostained with antibodies for the pan-neuronal transcriptionfactor Tlx3 and the peripheral nervous system filament protein Peripherin. Scale bar, 100mm.(C) DRGs ofPrdm12KO embryos contain fewer neurons than WT at E13.5. Each dot represents the mean value of Peripherin+cells in one biological replicate.Mann-Whitney test. *p = 0.029. Mean±SD.(D) Histogram of mean soma area of Peripherin+DRG neurons in E14.5 WT and KO embryos (WT = 1,273 cells; KO = 546 cells; DRG sections from three embryosof each genotype were used).(E) Increased number of apoptotic cells was observed at E12.5 inPrdm12KO embryos. Each dot represents the mean value of caspase-3+cells in one biologicalreplicate. Mann-Whitney test. E10.5, p = 0.300; E11.5, p = 0.484; E12.5, **p = 0.006. Mean±SD.(F) Cell proliferation is unaffected in DRG of E10.5 and E11.5 KO embryos. Each dot represents the mean value of pH3+cells in one biological replicate. Mann-Whitney test. E10.5, p > 0.999; E11.5, p > 0.999. Mean±SD.(G) Neuron number is unchanged in DRG of E11.5 WT andPrdm12KO embryos. Each dot represents the mean value of Islet1+cells in one biological replicate.Mann-Whitney test. p = 0.474. Mean±SD.(H) Transverse sections of DRG of E14.5 WT andPrdm12KO embryos processed by ISH forNtrk1,Ntrk2, andNtrk3(scale bar, 100mm). The number ofNtrk2- andNtrk3-expressing cells appears similar in E14.5 WT andPrdm12KO embryos, as estimated by counting their number per section, a method used here so as totake into consideration the reduced size of the DRG in KO embryos. Each dot represents the mean value of positive cells in one biological replicate. Mann-Whitney test.Ntrk2, p = 0.191;Ntrk3, p = 0.254. Mean±SD.(I) ISH on transverse sections of E14.5 WT and KO embryos showing the unaltered expression ofNtrk1in sympathetic ganglia. Scale bars, 100mm.(J) ISH on transverse sections of the head of E13.5 WT and KO embryos showing thatNtrk1is lost in the TG of KO embryos. Scale bar, 100mm.TG, trigeminal ganglion; DRG, dorsal root ganglion; SJG, superior-jugular ganglion; SC, spinal cord; SG sympathetic ganglion. SC, DRG, and SG are delineatedwith dashed lines. Image published in: Desiderio S et al. (2019) Copyright © 2019. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |