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Figure 7. PRDM12 Promotes a Nociceptor Fate in Human Embryonic Stem Cells Differentiated into sensory neurons (A) Transverse sections at the thoracic level of the spinal cord and DRG (delineated with dashed lines) of a CS12 human embryo stained with PRDM12 and TRKA antibodies. Lower panels are high magnifications of some PRDM12+/TRKA+ cells observed in the DRG (indicated by the yellow arrowhead). Scale bars, 25mm. (B) qRT-PCR analysis of the expression of PRDM12, NTRK1, NGN1, and NGN2in uninduced Prdm12-RMCE iPSCs differentiated into sensory neurons and harvested at day 7, 15, or 25of differentiation. Expression levels were compared to the level in undifferentiated iPSCs, defined as 1 (mean±SEM). (C) qRT-PCR analysis of the expression ofPRDM12, NTRK1, NTRK2, andNTRK3in doxycycline-induced Prdm12-RMCE iPSCs differentiated into sensory neurons and harvested until day 7, 15, or 25 of differentiation. Expression levels were compared to the expression level in uninduced control cells, which was defined as 1 (mean±SEM). (D) Immunostaining of TRKA and PRDM12 on Prdm12-RMCEiPSCs differentiated into sensory neurons and harvested at day 32. Scale bar, 25mm. See also Figure S7.In (B) and (C), individual values for biological replicates areindicated (dots). Image published in: Desiderio S et al. (2019) Copyright © 2019. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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