Xenbase Image ID: 175459

Fig. 3. Spatio-temporal expression pattern of Dpp4 in Xenopus early embryogenesis. (A) Temporal expression pattern of Dpp4 analyzed by RT-PCR. St., a developmental stage. ODC serves as a loading control. —RT, stage 27 control whole embryos in the absence of reverse transcriptase. (B and C) Whole mount in situ hybridization (B) and RT-PCR (C) showing spatial expression pattern of Dpp4 transcripts at stage 10.25. Embryos in (B) are shown in lateral (upper panel) or dorso-vegetal (lower panel) view with animal to the top. For (C), respective regional explants were dissected from stage 10.25 whole embryos. D, dorsal; V, ventral; VP, vegetal pole; MZ, marginal zone; AP, animal pole; DMZ, dorsal marginal zone; VMZ, ventral marginal zone. Chordin is a dorsal mesodermal marker, Vg1 is a member of TGF-β family expressed vegetally, and EF1-α is a loading control. —RT, stage 10.25 control whole embryos in the absence of reverse transcriptase. Image published in: Park DS et al. (2018) Copyright © 2018 by the The Korean Society for Biochemistry and Molecular Biology. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Gene Synonyms Species Stage(s) Tissue dpp4.L X. laevis Throughout NF stage 10.25 animal hemisphere marginal zone Image source: Published Larger Image Printer Friendly View

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