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Fig. 4. Embryonic morphological phenotypes caused by overexpression of Dpp4. (A) Four-cell stage embryos were injected in the dorsal or ventral marginal regions with Dpp4 (500 pg) mRNA and cultured to tadpole stages. Embryos are shown in lateral views with anterior to the left. Uninjected, uninjected control embryos. (B) Four-cell stage embryos were injected in the dorsal marginal region with LacZ (50 pg) alone or with Dpp4 (1 ng) mRNA, cultured to stage 10.25, and fixed for LacZ staining (red) and subsequent in situ hybridization against Goosecoid (Gsc). Arrows indicate expression of Gsc in the dorsal lip. Embryos are shown in dorso-vegetal views with anterior to the top. Control, an embryo injected with LacZ alone. Image published in: Park DS et al. (2018) Copyright © 2018 by the The Korean Society for Biochemistry and Molecular Biology. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license Gene Synonyms Species Stage(s) Tissue gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.25 dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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